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KMID : 0387720070180010032
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Korean Journal of Blood Transfusion
2007 Volume.18 No. 1 p.32 ~ p.38
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Evaluation of the DG Gel System using the Microtube Column Agglutination Technique for Antibody Screening and Identification
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Kim Jee-Yong
Huh Ji-Hoon
Kim Sun-Hyung
Nam Myung-Hyun
Roh Kyong-Ho
Kim Jang-Su
Bae Sook-Young
Yang Jin-Hyuk
Yoon Soo-Young
Lim Chae-Seung
Lee Chang-Kyu
Cho Yun-Jung
Kim Young-Kee
Lee Kap-No
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Abstract
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Background:For the antibody screening test, the classical LISS tube indirect antiglobulin test has been replaced by the microtube column agglutination system in Korea. This system was first created in 1990 by Lapierre and it is distributed through DiaMed (DiaMed Ag, Cresssier, Morat, Switzerland) around the world. Similar systems, such as Ortho BioVue, have been developed and competed after that. We evaluated a newly developed microtube column agglutination system, DG Gel (Diagnostic Grifols, Barcelona, Spain), and we compare it with the other established systems.
Method: In a comparative study, a total of 126 samples, including 76 antibody screening positive samples and 50 negative samples, were tested in parallel by the LISS/Coombs card (DiaMed Ag, Cresssier, Morat, Switzerland) and the DG Gel microtube column agglutination system. The positive samples that were proved by the LISS/Coombs card and the DG Gel system were identified by the ID-Dia panel (DiaMed Ag, Cresssier, Morat, Switzerland) and Identisera Diana (Diagnostic Grifols, Barcelona, Spain). Discrepant samples were rechecked with I, II and III cells that were supplied by the panel of the Korea Red Cross Blood Center.
Results:Among the 126 samples, the DG Gel antibody screening system showed 98.7% (75/76) sensitivity and 100% (50/50) specificity. We obtained concordant results in 75 samples (98.7%) and discrepant results in one sample (1.32%) between the DG Gel and DiaMed-ID for antibody identification.
Conclusions: Both the microtube column agglutination systems work well and showed high estimated sensitivity and specificity with high concordance. Therefore, the DG gel microtube column agglutination system can be used with good results.
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KEYWORD
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Antibody screening, Column agglutination, DG gel, DiaMed-ID
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